FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19216
Sequences flagged as poor quality0
Sequence length148-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT710936.995212323064116No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT539828.091174021648623No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGTGGT270614.082014987510409No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT2881.4987510407993339No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT2601.3530391340549544No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT2031.0564113238967527No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT1570.8170274771024147No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT730.37989175686927557No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT660.3434637801831807No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT640.33305578684429643No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGCGGT610.31744379683597No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT550.28621981681931724No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT520.2706078268109909No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT480.2497918401332223No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC480.2497918401332223No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGC440.2289758534554538No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCTGGAGTTTTGTGTGGT420.21856786011656953No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT400.20815986677768525No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTCTTGTGTGGT380.19775187343880102No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGCTTTGTGTGGT350.1821398834304746No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT340.17693588676103247No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT330.17173189009159034No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT320.16652789342214822No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT300.15611990008326393No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGCGTGCGGT290.15091590341382183No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTTGTGTGGT290.15091590341382183No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTTGGAGTTTTGTGTGGT290.15091590341382183No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAATTTTGTGTGGT280.14571190674437967No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTTGGAGTTTTGTGTGGT270.14050791007493754No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTCGTGCGGT270.14050791007493754No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGTGGC240.12489592006661115No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTTGGAGTTTTGTGTGGT240.12489592006661115No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT230.11969192339716901No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT230.11969192339716901No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT230.11969192339716901No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGTGTGTGGT220.1144879267277269No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTTGGAATTTTGTGTGGT210.10928393005828477No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT210.10928393005828477No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTTGGTGTTTTGTGTGGT200.10407993338884262No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAACTTTGTGTGGT200.10407993338884262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT159.478029E-5154.17451145
TAAATTT306.184564E-10144.940112
AATCTAT100.00704587144.940114
CTATAAG100.00704587144.940117
AAATCTA100.00704587144.940113
AAATTTA18950.0144.940113
ATAAGAT19100.0144.940119
AATTTAT18950.0144.940114
ATCTATA100.00704587144.940115
GAAAATT18600.0144.940111
GAAAATC100.00704587144.940111
TATAAGA19150.0144.940118
TCTATAA100.00704587144.940116
TATTTAT151.2134412E-4144.940114
AAAATTT18650.0144.940112
GAATATT151.2134412E-4144.940111
AATATTT151.2134412E-4144.940112
AAAATCT100.00704587144.940112
ATATTTA151.2134412E-4144.940113
TTTATAA19100.0144.940116
Produced by FastQC (version 0.11.5)