FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15965
Sequences flagged as poor quality0
Sequence length148-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT933058.44033823989978No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT245115.352333228938303No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT6704.1966802380206705No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT4352.7247103037895397No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT4132.5869088631381145No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT2071.2965862824929533No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT1971.233949264015033No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT940.5887879736924523No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT840.5261509552145317No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT570.35703100532414656No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT540.33823989978077046No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT510.3194487942373943No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT510.3194487942373943No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC440.27560288130285No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT430.2693391794550579No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT390.24428437206388975No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT370.23175696836830567No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGCGTGCGGT270.16911994989038523No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTCGTGCGGT260.1628562480425932No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT250.15659254619480112No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTCGTGCGGT250.15659254619480112No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT240.1503288443470091No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTTGTGCGGT220.137801440651425No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTCGTGCGGT200.1252740369558409No Hit
GAAAATTTATAAGATTGCGTAAAGTAGTAAGGTTCGGTGTTTCGTGCGGT200.1252740369558409No Hit
GAAAATTTATAAGACTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT190.11901033510804886No Hit
GAAAATTTATAAGATTGCGTAAAGCTGTAAGGTTCGGAGTTTCGTGCGGT180.1127466332602568No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT180.1127466332602568No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTTGTGCGGT170.10648293141246477No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT170.10648293141246477No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT160.10021922956467272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT201.625931E-6152.10526145
TAAATTT151.20917255E-4144.952992
AGGACTT151.20917255E-4144.952999
GAAAATT15400.0144.952991
AAAATTT15400.0144.952992
GTAAATT151.20917255E-4144.952991
GTTGACA202.069648E-6144.952971
TAGGACT202.069648E-6144.952978
TGACATA202.069648E-6144.952973
ATAGGAC202.069648E-6144.952977
TATAAAA100.0070328577144.952978
TTATAAA100.0070328577144.952977
GACATAG202.069648E-6144.952974
ACATAGG202.069648E-6144.952975
CATAGGA202.069648E-6144.952976
AATTTAT15650.0144.489854
TTTATAA15650.0144.489856
ATTTATA15650.0144.489855
AAATTTA15600.0144.488393
TATAAGA15600.0144.488398
Produced by FastQC (version 0.11.5)