FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15454
Sequences flagged as poor quality0
Sequence length148-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT876856.736120098356416No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT294419.05008412061602No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT5173.3454121910185064No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT3462.238902549501747No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT2241.4494629222207842No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT1651.067684741814417No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT830.5370777792157371No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT830.5370777792157371No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT790.5111945127475087No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT760.4917820628963375No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT670.4335447133428239No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT590.3817781804063673No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT470.30412838100168244No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT440.2847159311505112No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC420.271774297916397No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT380.24589103144816876No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT360.2329493982140546No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT350.22647858159699752No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT340.22000776497994048No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT320.20706613174582633No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTCGTGCGGT310.20059531512876924No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTCTTGTGTGGT270.17471204866054096No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTCGTGCGGT260.1682412320434839No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGC230.14882878219231266No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGCTTTGTGTGGT220.1423579655752556No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTCGTGCGGT210.1358871489581985No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGCGTGCGGT210.1358871489581985No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT200.12941633234114144No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGCGCGGT200.12941633234114144No Hit
GAAAATTTATAAGATTACGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT190.12294551572408438No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT190.12294551572408438No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT190.12294551572408438No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAGTTTCGTGCGGT190.12294551572408438No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTCTGTGTGGT180.1164746991070273No Hit
GAAAATTTATAAGATTGCGTAAAGTTGCAAGGTTCGGAGTTTCGTGCGGT170.11000388248997024No Hit
GAAAATTTATAAGACTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT160.10353306587291317No Hit
GAAAATTTATAAGATTGCGTAAGGTTGTAAGGTTCGGAGTTTCGTGCGGT160.10353306587291317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCTT100.0059339693153.38356145
GAAAATT14800.0144.944991
TAAATTT202.0681218E-6144.944982
GTAAATT202.0681218E-6144.944981
GTCTCTT14650.0144.48418145
ATAAGAT14950.0144.460229
AAAATTT14900.0144.458592
AAATTTA15150.0143.988253
AATTTAT15150.0143.988254
ATTTATA15150.0143.988255
TTTATAA15100.0143.985096
TATAAGA15050.0143.981898
TTATAAG15050.0143.981897
TGTTGTA4600.028.98899890-94
CGGGGGT200.006051528.988998130-134
ACGTTAC6750.028.98899885-89
TATGTTG4700.028.98899890-94
GGGTAGC200.006051528.988998135-139
GTCGTAT800.028.98899890-94
GTTTTCG200.006051528.988998100-104
Produced by FastQC (version 0.11.5)