FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15454
Sequences flagged as poor quality0
Sequence length135-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG375724.310858030283423No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG336821.79371036624822No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA262516.985893619774817No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA5583.610715672317846No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA3272.1159570337776628No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG2401.5529959880936974No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACA1911.2359259738579007No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG1460.9447392260903326No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG1280.8282645269833053No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG1260.8153228937491911No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG1250.808852077132134No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG1170.7570855441956774No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG1100.711789827876278No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACA1060.6859065614080496No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACAACG920.5953151287692506No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCACTAAAACGTACGACG880.5694318623010224No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG690.446486346576938No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA690.446486346576938No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA540.34942409732108193No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA530.3429532807040248No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA390.2523618480652258No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACAACA390.2523618480652258No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACA380.24589103144816876No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG370.23942021483111167No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAGAACGTACGACG350.22647858159699752No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG340.22000776497994048No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG340.22000776497994048No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG330.2135369483628834No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG330.2135369483628834No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTGAAACATACAACA310.20059531512876924No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACGACG310.20059531512876924No Hit
CTGACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA300.19412449851171218No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG280.18118286527759803No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACA270.17471204866054096No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAGCGTACGACG260.1682412320434839No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGGCG230.14882878219231266No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAGCA230.14882878219231266No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG220.1423579655752556No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAGACGTACGACG220.1423579655752556No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTGCGACG220.1423579655752556No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG210.1358871489581985No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACA210.1358871489581985No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT190.12294551572408438No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCACTAAAACGTACGACG190.12294551572408438No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG180.1164746991070273No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTGAAACGTACGACG170.11000388248997024No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG170.11000388248997024No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG170.11000388248997024No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAGACATACAACA170.11000388248997024No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG160.10353306587291317No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG160.10353306587291317No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCACTAAAACGTACGACA160.10353306587291317No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG160.10353306587291317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCC306.0936145E-10144.948232
GAAGCAA151.2085976E-4144.948239
CGAAGCA151.2085976E-4144.948238
CTGACCC306.0936145E-10144.948231
GACCCCG306.0936145E-10144.948233
CCGAAGC151.2085976E-4144.948237
CCCAGAG100.0070314184144.948215
GAAACAA15050.0144.948219
AACCCAG100.0070314184144.948213
TAACCCC14850.0144.948212
CTAACCC14950.0144.948211
CCCGAAG100.0070314184144.948216
CCCGAAA15000.0144.948216
CGAAACA15000.0144.948218
ACCCAGA100.0070314184144.948214
GAGACAA100.0070314184144.948219
CCGAAAC15000.0144.465047
GTCTCTT14900.0144.14308145
ACCCCGA15300.0144.000844
CCCCGAA15250.0143.997745
Produced by FastQC (version 0.11.5)