FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17821
Sequences flagged as poor quality0
Sequence length148-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT822646.15902586835755No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT495427.79866449694181No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT6883.860613882498176No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT5212.923517198810392No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT4342.435329106110768No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT2801.5711800684585602No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT2501.4028393468380003No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT920.516244879636384No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT730.409629089276696No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT700.39279501711464004No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT660.37034958756523206No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT580.325458728466416No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC510.286179226754952No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT430.241288367656136No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT380.21323158071937603No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT330.18517479378261603No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT320.179563436395264No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT300.16834072162056002No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGCGTGCGGT280.15711800684585603No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGC280.15711800684585603No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT280.15711800684585603No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTTGGAGTTTTGTGTGGT270.151506649458504No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT260.145895292071152No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT260.145895292071152No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTCTGTGTGGT230.129061219909096No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGGTTTGTGTGGT230.129061219909096No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGCTTTGTGTGGT220.123449862521744No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTCTTGTGTGGT210.11783850513439201No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTTGGAGTTTTGTGTGGT210.11783850513439201No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGTGTGTGGT210.11783850513439201No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCTGGAGTTTTGTGTGGT210.11783850513439201No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTTGGAGTTTTGTGTGGT210.11783850513439201No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGCGTGGT200.11222714774704No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT190.10661579035968802No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT180.10100443297233601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAATT17450.0144.980331
AATTTAT17650.0144.569644
ATTTATA17650.0144.569645
AAATTTA17600.0144.568473
TTTATAA17600.0144.568476
ATAAGAT17550.0144.567289
TATAAGA17550.0144.567288
TTATAAG17550.0144.567287
AAAATTT17550.0144.567282
GTCTCTT17350.0144.05254145
TTTGACG8800.028.99606980-84
TAGCGAG10200.028.996069105-109
GTTTGAC8800.028.99606980-84
CGTTATG552.0736479E-1028.99606985-89
ACGTTTT10200.028.99606995-99
TTCGTGT403.2022217E-728.99606940-44
TTCGTGC9600.028.99606940-44
TTGTACG304.358429E-528.99606995-99
ACGTCGT8800.028.99606990-94
CGGGGGT304.358429E-528.996069130-134
Produced by FastQC (version 0.11.5)