FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15711
Sequences flagged as poor quality0
Sequence length150-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT878355.903507096938455No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGTGGT186011.838839029978995No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT184911.76882439055439No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT2881.8331105594806187No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT1971.2538985424225066No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT1601.0183947552670105No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT1330.8465406403157023No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT1070.6810514925848132No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC1010.6428616892623004No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT940.5983069187193686No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT580.36916809878429124No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT520.33097829546177837No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT500.3182483610209408No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTCGTGCGGT440.28005855769842786No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGCGGT380.24186875437591498No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT350.2227738527146585No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT340.2164088854942397No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT330.2100439182738209No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT320.20367895105340206No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT310.19731398383298326No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGCGCGGT300.19094901661256444No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT260.16548914773088919No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT240.15275921329005157No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT240.15275921329005157No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTCGTGCGGT220.14002927884921393No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGCGTGCGGT200.1272993444083763No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT190.12093437718795749No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT190.12093437718795749No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTCGTGCGGT180.11456940996753867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCTT100.0063169077150.22772145
GTATCAT100.0063169077150.22772145
GTCCCTT100.0063169077150.22772145
ATAAGAT15450.0144.964989
TATAAGA15450.0144.964988
GTTGACA100.0070300708144.964971
TAGGACT100.0070300708144.964978
AAATTTA15400.0144.964973
AGGACTT100.0070300708144.964979
AATTTAT15400.0144.964974
GAAAATT15500.0144.964971
TGACATA100.0070300708144.964973
ATAGGAC100.0070300708144.964977
TTATAAG15400.0144.964977
GACATAG100.0070300708144.964974
ACATAGG100.0070300708144.964975
TTGACAT100.0070300708144.964972
CATAGGA100.0070300708144.964976
AAAATTT15500.0144.964972
TTTATAA15400.0144.964976
Produced by FastQC (version 0.11.5)