FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15711
Sequences flagged as poor quality0
Sequence length146-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG799150.862453058366754No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA327220.82617274521036No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG2211.4066577557125581No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG1911.2157087390999937No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG1851.177518935777481No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG1801.1456940996753868No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG1671.0629495258099422No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG1550.9865699191649162No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA1250.7956209025523517No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA1030.655591623703138No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG910.5792120170581122No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG820.5219273120743428No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG800.5091973776335053No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG680.4328177709884794No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA650.4137228693272229No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA530.33734326268219716No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG490.31188339380052194No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG440.28005855769842786No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG440.28005855769842786No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA420.2673286232575902No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG360.22913881993507734No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG350.2227738527146585No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG280.1782190821717268No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT260.16548914773088919No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG260.16548914773088919No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG250.1591241805104704No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTGAAACATACAACA240.15275921329005157No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG240.15275921329005157No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG230.14639424606963275No Hit
CTAACCCCGAAACAACCAAACCCCACACACCCTCACTAAAACATACAACA230.14639424606963275No Hit
CTGACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA220.14002927884921393No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAGCATACAACA220.14002927884921393No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG220.14002927884921393No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG210.1336643116287951No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG210.1336643116287951No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG200.1272993444083763No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG200.1272993444083763No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCGCGCTAAAACGTACGACG190.12093437718795749No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG180.11456940996753867No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACA180.11456940996753867No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAGACATACAACA180.11456940996753867No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACG170.10820444274711985No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG170.10820444274711985No Hit
CTAACCCCGAAACAACCAACTCCACACACCCTCACTAAAACATACAACAT170.10820444274711985No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAGCA160.10183947552670103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG100.007029147144.971345
GCCCAGA100.007029147144.971344
GAAACAA15100.0144.971349
TGACCCC100.007029147144.971342
ATGGCCC100.007029147144.971341
GGCCCAG100.007029147144.971343
CCCCGAG100.007029147144.971345
CCCGAGA100.007029147144.971346
CCGAGAC100.007029147144.971347
CGAGACA100.007029147144.971348
GACCCCG100.007029147144.971343
TGGCCCA100.007029147144.971342
CCGAAAA100.007029147144.971347
TAACCCC15350.0144.971332
CTAACCC15350.0144.971331
CCCGAAA15300.0144.971336
CGAAACA15150.0144.971338
GAGACAA151.2082023E-4144.971339
CCGAAAC15200.0144.971337
GTCTCTT15400.0144.888145
Produced by FastQC (version 0.11.5)