EGAF00005323627 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005323627
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16434
Sequences flagged as poor quality	0
Sequence length	149-151
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTCGTGCGGT	8215	49.98783010831204	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	2297	13.977120603626627	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGTGGT	2227	13.551174394547887	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTTGTGCGGT	347	2.11147620786175	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT	285	1.7342095655348666	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTCGTGCGGT	227	1.3812827065839113	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTCGTGTGGT	133	0.8092977972496045	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT	95	0.5780698551782889	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTCGTGCGGC	69	0.4198612632347572	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAATTTCGTGCGGT	67	0.4076913715467932	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAATTTCGTGCGGT	65	0.39552147985882924	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAATTTCGTGCGGT	56	0.3407569672629914	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAACTTCGTGCGGT	55	0.33467202141900937	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTCCGTGCGGT	53	0.32250212973104536	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT	53	0.32250212973104536	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATCTCGTGCGGT	52	0.3164171838870634	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGACTTTCGTGCGGT	51	0.3103322380430814	No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAATTTCGTGCGGT	47	0.28599245466715345	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTTGTGCGGT	46	0.27990750882317145	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTTGTGTGGT	40	0.24339783375927956	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGATTTCGTGCGGT	32	0.19471826700742365	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAATTTCGTGCGGT	32	0.19471826700742365	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT	30	0.18254837531945967	No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTAGGGCGTGCGGT	28	0.1703784836314957	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAATTTCGTGCGGT	28	0.1703784836314957	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTCGCGCGGT	24	0.14603870025556773	No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAATTTCGTGCGGT	24	0.14603870025556773	No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAATTTCGTGCGGT	24	0.14603870025556773	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAATTTTGTGTGGT	23	0.13995375441158572	No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAATTTCGTGCGGT	23	0.13995375441158572	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAATTTCGTGTGGT	22	0.13386880856760375	No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTATTTCGTGCGGT	21	0.12778386272362174	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGCAAGGTTCGGAATTTCGTGCGGT	18	0.10952902519167579	No Hit
GAAAATTTATAAGATTGCGTAAAGTCGTAAGGTTCGGAATTTCGTGCGGT	17	0.1034440793476938	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT	17	0.1034440793476938	No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	17	0.1034440793476938	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAT	15	9.653535E-5	153.3613	145
TCTCTTT	10	0.005940074	153.3613	145
GTTGACA	10	0.007035803	144.94513	1
TAGGACT	15	1.2100555E-4	144.94513	8
AGGACTT	15	1.2100555E-4	144.94513	9
GAAAATT	1590	0.0	144.94513	1
TGACATA	15	1.2100555E-4	144.94513	3
ATAGGAC	15	1.2100555E-4	144.94513	7
GACATAG	15	1.2100555E-4	144.94513	4
ACATAGG	15	1.2100555E-4	144.94513	5
TTGACAT	15	1.2100555E-4	144.94513	2
CATAGGA	15	1.2100555E-4	144.94513	6
AAAATTT	1595	0.0	144.94513	2
AAATTTA	1605	0.0	144.49358	3
ATAAGAT	1600	0.0	144.49217	9
AATTTAT	1600	0.0	144.49217	4
TATAAGA	1600	0.0	144.49217	8
TTATAAG	1595	0.0	144.49075	7
TTTATAA	1595	0.0	144.49075	6
ATTTATA	1595	0.0	144.49075	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content