FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434
Sequences flagged as poor quality0
Sequence length132-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG775647.1948399659243No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA387923.603504928806135No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG2091.2717536813922357No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG2061.2534988438602896No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA1891.1500547645125958No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG1831.113545089448704No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG1671.0161859559449922No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG1520.9249117682852623No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG1420.8640623098454423No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA1180.7180236095898747No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG1000.6084945843981989No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG740.45028599245466716No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA700.4259462090787392No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG570.3468419131069734No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG550.33467202141900937No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA530.32250212973104536No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG510.3103322380430814No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT490.29816234635511746No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA470.28599245466715345No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG460.27990750882317145No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG380.23122794207131558No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG340.2068881586953876No Hit
CTGACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA330.2008032128514056No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG330.2008032128514056No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG320.19471826700742365No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG320.19471826700742365No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG300.18254837531945967No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG300.18254837531945967No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG280.1703784836314957No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACA280.1703784836314957No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG280.1703784836314957No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATGCAACA250.15212364609954973No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAGACATACAACA240.14603870025556773No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACA240.14603870025556773No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTGAAACATACAACA230.13995375441158572No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAGAACATACAACA230.13995375441158572No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG230.13995375441158572No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAATCGTACGACG230.13995375441158572No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG210.12778386272362174No Hit
CTAACCCCGAAACAACAAACTCCGCACGCCCTCGCTAAAACGTACGACGT210.12778386272362174No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAGCA200.12169891687963978No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACATACAACA200.12169891687963978No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACG190.11561397103565779No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACAACG180.10952902519167579No Hit
CTAACCCCGAAACAACCAAACCCCACACACCCTCACTAAAACATACAACA180.10952902519167579No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCCCACTAAAACATACAACA170.1034440793476938No Hit
CTAACCCCGAAACAACCAAATCCGCACGCCCTCGCTAAAACGTACGACGT170.1034440793476938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGA100.007035361144.948174
GAGGCAA202.071767E-6144.948179
AATCCCG100.007035361144.948173
AGGGCTC151.2099543E-4144.948171
AGAGGCA202.071767E-6144.948178
CCCCGAG100.007035361144.948175
GAACCCC151.2099543E-4144.948172
CTAACCC15550.0144.948171
TCAGAGG151.2099543E-4144.948176
CGAACCC202.071767E-6144.948171
CTAATCC100.007035361144.948171
GGCTCAG151.2099543E-4144.948173
TAATCCC100.007035361144.948172
CTCAGAG202.071767E-6144.948175
CCGAGAC100.007035361144.948177
TCCCGAA100.007035361144.948175
CGAGACA100.007035361144.948178
GCTCAGA151.2099543E-4144.948174
GAGACAA151.2099543E-4144.948179
CCGAAAA100.007035361144.948177
Produced by FastQC (version 0.11.5)