FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15647
Sequences flagged as poor quality0
Sequence length132-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG987063.079184508212435No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA15139.669585224004601No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG2641.6872243880616093No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA2081.3293283057455103No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG2051.3101553013357192No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG1811.1567712660573912No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG1811.1567712660573912No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG1701.0864702498881575No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG1540.984214226369272No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG870.5560171278839394No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG770.492107113184636No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG660.42180609701540234No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG600.3834600881958203No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG550.3515050808461686No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG540.3451140793762383No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG530.3387230779063079No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG500.3195500734965169No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA410.26203106026714384No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG390.2492490573272832No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG380.24285805585735287No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG370.2364670543874225No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG330.21090304850770117No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT330.21090304850770117No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG290.18533904262797982No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA260.1661660382181888No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG250.15977503674825844No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG250.15977503674825844No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT250.15977503674825844No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA220.14060203233846744No Hit
CTAACCCCGAAACAATCAAACTCCGCACGCCCTCGCTAAAACGTACGACG220.14060203233846744No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG200.12782002939860676No Hit
CTAACCCCGAAACAACAAACTCCGCACGCCCTCGCTAAAACGTACGACGT200.12782002939860676No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG190.12142902792867644No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG190.12142902792867644No Hit
CTAACCCCGAAACACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT190.12142902792867644No Hit
CTAACCCCGAAACAACCAAGCTCCGCACGCCCTCGCTAAAACGTACGACG190.12142902792867644No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG170.10864702498881575No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAATCGTACGACG170.10864702498881575No Hit
CTAACCCTGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG170.10864702498881575No Hit
TTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG170.10864702498881575No Hit
CTAACCCCGAAACAACCAAACTCCGCGCGCCCTCGCTAAAACGTACGACG160.10225602351888541No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCGCGCTAAAACGTACGACG160.10225602351888541No Hit
CGAACCCCGAAACAACCAAACTCCGCACGCCCGCGCTAAAACGTACGACG160.10225602351888541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCTT201.6430677E-6151.7651145
GTCTCTT15000.0145.18861145
CTGACCC151.2085927E-4144.955141
GAGACAA151.2085927E-4144.955149
TGACCCC202.0680436E-6144.955122
GAAGCAA100.007031069144.955129
CGAAGCA100.007031069144.955128
CCCCGAG100.007031069144.955125
CCCGAGA100.007031069144.955126
CCCGAAG100.007031069144.955126
CTCAGAG100.007031069144.955125
CCGAGAC100.007031069144.955127
CGAGACA100.007031069144.955128
GACCCCG202.0680436E-6144.955123
CTAACCC14950.0144.470321
TAACCCC15050.0143.991972
ACCCCGA15300.0143.5344
CCCCGAA15200.0143.524645
GAAACAA15200.0143.047829
CGAAACA15200.0143.047828
Produced by FastQC (version 0.11.5)