FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13164
Sequences flagged as poor quality0
Sequence length150-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT601645.70039501671225No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT191414.53965360072926No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT165412.564570039501671No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT6334.808568824065634No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT4703.5703433606806447No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT2752.089030689759951No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT1601.2154360376785172No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT750.5697356426618049No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT610.4633849893649347No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT610.4633849893649347No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT510.38742023701002737No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT390.29626253418413856No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT380.28866605894864783No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT340.2582801580066849No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC330.2506836827711942No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT300.22789425706472194No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT290.22029778182923124No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT270.20510483135824978No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGCGCGGT220.1671224551807961No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT220.1671224551807961No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT220.1671224551807961No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGCGTGCGGT210.15952597994530537No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT200.15192950470981464No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTCGTGCGGT190.14433302947432392No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT190.14433302947432392No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTTGTGCGGT160.12154360376785171No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT150.11394712853236097No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTCGTGCGGT150.11394712853236097No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT140.10635065329687024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT100.006396155149.5098145
GAAAATT12950.0145.515271
AAAATTT12950.0145.515272
AAATTTA13050.0144.961983
ATAAGAT13050.0144.961989
AATTTAT13050.0144.961984
TATAAGA13100.0144.961988
TTTATAA13050.0144.961986
ATTTATA13050.0144.961985
GTCTCTT12900.0144.87384145
TTATAAG13150.0144.410787
TTTTGTG4850.028.99239740-44
TTGTTTT2450.028.99239770-74
TGGTTTT3050.028.99239760-64
TTTGACG8800.028.99239780-84
TTCGTGT255.1101274E-428.99239740-44
ACGTTGT552.0372681E-1028.99239790-94
TATGTTG2000.028.99239790-94
GCGGTTA10150.028.99239745-49
TTTTTCG10250.028.99239765-69
Produced by FastQC (version 0.11.5)