FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005323632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13164
Sequences flagged as poor quality0
Sequence length135-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG529640.23093284715892No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA132410.05773321178973No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG7845.9556365846247346No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG7325.560619872379216No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG3672.787906411425099No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG3482.643573381950775No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG2702.0510483135824975No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA2391.815557581282285No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG1851.4053479185657853No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG1411.0711030082041932No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG1190.9039805530233971No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG1000.7596475235490732No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG900.683682771194166No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACG660.5013673655423884No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA550.4178061379519903No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG530.40261318748100877No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACA510.38742023701002737No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG510.38742023701002737No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA440.3342449103615922No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA390.29626253418413856No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG390.29626253418413856No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG370.2810695837131571No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG370.2810695837131571No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG360.27347310847766637No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA330.2506836827711942No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACAACG310.2354907323002127No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG300.22789425706472194No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACA290.22029778182923124No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCACTAAAACGTACGACG270.20510483135824978No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACG260.19750835612275902No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACATACGACG260.19750835612275902No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA250.1899118808872683No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG230.17471893041628683No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG230.17471893041628683No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACA220.1671224551807961No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT220.1671224551807961No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG220.1671224551807961No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG220.1671224551807961No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACATACAACA210.15952597994530537No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG200.15192950470981464No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG200.15192950470981464No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACAACG200.15192950470981464No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG190.14433302947432392No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACA180.13673655423883319No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACGACG180.13673655423883319No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA180.13673655423883319No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG170.12914007900334246No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACAACG160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACA160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACA160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACA160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACAACA160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAATCGTACGACG160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAGACATACAACA160.12154360376785171No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACG150.11394712853236097No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG140.10635065329687024No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACG140.10635065329687024No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG140.10635065329687024No Hit
CTAACCCCGAAACAACCAAACTCCGCGCGCCCTCGCTAAAACGTACGACG140.10635065329687024No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACATACGACG140.10635065329687024No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACA140.10635065329687024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)