FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17586
Sequences flagged as poor quality0
Sequence length132-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG321118.258842260889345No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG181910.343455021039464No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG12146.903218469236894No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA9425.356533606277721No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA9195.225747753895144No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA8895.055157511657No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG7594.315933128625042No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACA5272.9967019219833957No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG4412.5076765609007166No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG3852.189241442056181No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACA3572.0300238826339134No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA3532.0072785170021605No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACG3091.757079495052883No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACA2561.4557034004321618No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA1550.8813829182304106No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACA1510.858637552598658No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG1200.6823609689525759No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACAACA1180.6709882861366996No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACA1150.6539292619128853No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACA910.51745706812237No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG830.47196633685886497No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG760.4321619470032981No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACGACG750.4264756055953599No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG670.3809848743318549No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG660.37529853292391674No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACA600.34118048447628796No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG600.34118048447628796No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACATACGACA590.3354941430683498No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACA510.29000341180484474No Hit
CTAACCCCGAAACAACCAGACTCCGCACACCCTCGCTAAAACGTACGACG510.29000341180484474No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACA490.2786307289889685No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACGACA450.255885363357216No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACA420.2388263391334016No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA420.2388263391334016No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACAACA400.22745365631752532No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG380.21608097350164904No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACAACG370.2103946320937109No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG350.19902194927783465No Hit
CTAACCCCGAAACAACCAGACTCCGCACACCCTCACTAAAACGTACGACG340.1933356078698965No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACG310.17627658364608212No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACA310.17627658364608212No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACG310.17627658364608212No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACATACGACG290.16490390083020584No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG280.1592175594222677No Hit
CTAACCCCGAAACAACCAGACTCCGCACACCCTCGCTAAAACGTACGACA280.1592175594222677No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA260.14784487660639145No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACA260.14784487660639145No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG250.1421585351984533No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACAACG240.1364721937905152No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACAACA240.1364721937905152No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACAACG240.1364721937905152No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG240.1364721937905152No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACA230.13078585238257706No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTGCGACA230.13078585238257706No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA230.13078585238257706No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG230.13078585238257706No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACAACA220.12509951097463892No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACAACA220.12509951097463892No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG210.1194131695667008No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG200.11372682815876266No Hit
CTGACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG200.11372682815876266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTC151.21018675E-4144.982851
TGACCCC202.0732605E-6144.982852
GGACTCA151.21018675E-4144.982852
GAAGCAA100.0070343516144.982859
TAGCCCC100.0070343516144.982852
AACCCTG100.0070343516144.982853
CGAAGCA100.0070343516144.982858
CCTGAAA100.0070343516144.982856
AACCCGA100.0070343516144.982853
ACCCGAA100.0070343516144.982854
CTGACCC202.0732605E-6144.982851
ATAACCC100.0070343516144.982851
CAGAGAC151.21018675E-4144.982857
TAACCCT100.0070343516144.982852
TAACCCG100.0070343516144.982852
CCCCGAG100.0070343516144.982855
GAACCCC253.561945E-8144.982852
TCAGAGA151.21018675E-4144.982856
CGAACCC253.561945E-8144.982851
CCCGAGA100.0070343516144.982856
Produced by FastQC (version 0.11.5)