FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17329
Sequences flagged as poor quality0
Sequence length148-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1046960.41318021813146No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA206411.910669975186105No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG2701.558081828149345No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA2401.38496162502164No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG2051.1829880547059841No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG1851.0675745859541808No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG1811.0444918922038202No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG1730.9983265047030988No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG1710.9867851578279185No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG1160.6693981187604593No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG1030.5943793640717873No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG760.4385711812568527No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG730.42125916094408217No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG710.40971781406890184No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG660.380864446880951No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG570.3289283859426395No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA540.311616365629869No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA490.2827629984419182No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG480.276992325004328No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG420.24236828437878702No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG390.22505626406601648No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG370.21351491719083615No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA350.20197357031565585No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG350.20197357031565585No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG340.19620289687806566No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT330.1904322234404755No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACA290.16734952969011482No Hit
CTAACCCCGAAACAACCAAGCTCCGCACGCCCTCGCTAAAACGTACGACG240.138496162502164No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG230.13272548906457382No Hit
CTAGCCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG220.12695481562698366No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT220.12695481562698366No Hit
CGAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG220.12695481562698366No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA210.12118414218939351No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAATCGTACGACG210.12118414218939351No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAGCA210.12118414218939351No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG200.11541346875180335No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG190.10964279531421317No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG190.10964279531421317No Hit
CTAACCCCGAAACAACAAACTCCGCACGCCCTCGCTAAAACGTACGACGT190.10964279531421317No Hit
CTAACCCCGAAACAACCAAATCCGCACGCCCTCGCTAAAACGTACGACGT190.10964279531421317No Hit
CTAACCCTGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG190.10964279531421317No Hit
TTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG190.10964279531421317No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG180.103872121876623No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACA180.103872121876623No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCGCGCTAAAACGTACGACG180.103872121876623No Hit
CTAACCCCGAAACAGCCAAACTCCGCACGCCCTCGCTAAAACGTACGACG180.103872121876623No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG180.103872121876623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA100.0070383144.950876
CGACACA100.0070383144.950878
GAGGCAA100.0070383144.950879
AACGCCG100.0070383144.950873
TGACCCC151.211012E-4144.950872
CCTCGAA100.0070383144.950875
GAAGCAA100.0070383144.950879
AGAGGCA100.0070383144.950878
TCGAAAC100.0070383144.950877
CGAAGCA100.0070383144.950878
AACCTCG100.0070383144.950873
CTGACCC151.211012E-4144.950871
CAGAGGC100.0070383144.950877
GACACAA100.0070383144.950879
CCCCGAG151.211012E-4144.950875
GAACCCC151.211012E-4144.950872
CTAACGC100.0070383144.950871
CTAACCC16400.0144.950871
CGAACCC202.0748557E-6144.950871
CCCGAGA151.211012E-4144.950876
Produced by FastQC (version 0.11.5)