FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12502
Sequences flagged as poor quality0
Sequence length148-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT407732.610782274836026No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT322525.795872660374343No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT248319.86082226843705No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT3082.463605823068309No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT2461.9676851703727405No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT1261.007838745800672No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT1240.9918413053911375No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT620.49592065269556873No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT450.35994240921452564No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTTGTGCGGT380.303951367781155No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCTGTGCGGT370.2959526475763878No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTTGTGCGGT340.2719564869620861No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT340.2719564869620861No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT320.2559590465525516No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTTGGAGTTTTGTGTGGT260.20796672532394817No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT250.19996800511918092No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTCTGTGTGGT240.1919692849144137No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT240.1919692849144137No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGC240.1919692849144137No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT240.1919692849144137No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGCTTTGTGTGGT230.18397056470964646No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGC220.17597184450487924No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTTGTGCGGT200.15997440409534475No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTTGTGCGGT190.1519756838905775No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTTGTGCGGT180.14397696368581028No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT180.14397696368581028No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT170.13597824348104304No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGCGTGGT160.1279795232762758No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC160.1279795232762758No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTTGTGCGGT140.11198208286674133No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT140.11198208286674133No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT140.11198208286674133No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGTGTGCGGT130.10398336266197408No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTTGTGCGGT130.10398336266197408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATT100.006282139150.3776145
AAATTTA12350.0144.9643
ATAAGAT12450.0144.9649
AATTTAT12350.0144.9644
GAAAATT12300.0144.9641
TATAAGA12450.0144.9648
GAAGATT100.0070131114144.9641
GATTTAT100.0070131114144.9644
AAAATTT12300.0144.9642
AGATTTA100.0070131114144.9643
TTTATAA12450.0144.9646
ATTTATA12450.0144.9645
TTATAAG12400.0144.963997
GTCTCTT12200.0144.21457145
CGTTTGA9250.028.99280280-84
GTATGTT3050.028.99280295-99
GTTTGGA3150.028.99280230-34
GTTTGAT3050.028.99280280-84
GGGTTAG12200.028.992802135-139
CGTTACG8850.028.99280285-89
Produced by FastQC (version 0.11.5)