FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005323639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34336
Sequences flagged as poor quality0
Sequence length147-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT2451471.39445479962721No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT24147.030521901211556No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT12953.771551724137931No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT10833.154123951537745No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT2470.7193616029822926No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTTGTGCGGT1980.5766542404473438No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTTGTGCGGT1730.5038443616029823No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCTGTGCGGT1700.4951071761416589No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGC1530.44559645852749297No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTTGTGCGGT1480.43103448275862066No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTTGTGCGGT970.28250232991612306No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT950.2766775396085741No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTTGTGCGGT880.25629077353215285No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT840.244641192917055No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT830.24172879776328052No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTTGTGCGGT730.21260484622553588No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAGTTTTGTGCGGT700.20386766076421248No Hit
GAAAATTTATAAGATTGCGTAAAGCTGTAAGGTTCGGAGTTTTGTGCGGT670.1951304753028891No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTTGTGCGGT660.19221808014911462No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGA580.16891891891891891No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT570.16600652376514446No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGG550.1601817334575955No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGCGCGGT520.15144454799627213No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTTGTGCGGT500.1456197576887232No Hit
GAAAATTTATAAGATTGTGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT480.13979496738117428No Hit
GAAAATTTATAAGATTGCGTAAAGATGTAAGGTTCGGAGTTTTGTGCGGT460.13397017707362535No Hit
GAAAATTTATAAGATTGCGTAAAGTTGCAAGGTTCGGAGTTTTGTGCGGT410.11940820130475302No Hit
GAAAATTTATAAGATTACGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT370.10775862068965517No Hit
GAAAATTTATAAGATTGCGTAAGTTGTAAGGTTCGGAGTTTTGTGCGGTT370.10775862068965517No Hit
GAAAATTTATAAAATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT360.1048462255358807No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGATTTGTGCGGT350.10193383038210624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)