FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34336
Sequences flagged as poor quality0
Sequence length148-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG754921.98567101584343No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG680319.81302423112768No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG517915.083294501397951No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACA14694.2783084808946885No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG12783.722041006523765No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG9162.667753960857409No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG7482.178471575023299No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACG6181.7998602050326187No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACA5501.6018173345759552No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA4361.2698042870456663No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACAACG3801.1067101584342964No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACAACG3681.0717614165890028No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG3180.9261416589002796No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG3120.9086672879776329No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACA2800.8154706430568499No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG2400.6989748369058714No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACATACGACG2220.646551724137931No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG1950.5679170549860205No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACA1530.44559645852749297No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG1260.36696178937558244No Hit
CTCACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG1200.3494874184529357No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACA1170.3407502329916123No Hit
CTCACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1120.32618825722274No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAGCGTACGACG1030.2999767008387698No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG890.2592031686859273No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA840.244641192917055No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACG820.23881640260950604No Hit
CTCACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG780.2271668219944082No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACG770.22425442684063374No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTGAAACGTACGACG730.21260484622553588No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACG650.18930568499534017No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACATACGACA640.1863932898415657No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTGCGACG620.18056849953401677No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACAACA590.17183131407269336No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAGACGTACGACG570.16600652376514446No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG560.16309412861137No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTGAAACGTACGACG510.14853215284249766No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG490.14270736253494873No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGGCG470.1368825722273998No Hit
CTAACCCCGAAACAACCAACTCCACACGCCCTCGCTAAAACGTACGACGT430.12523299161230197No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG430.12523299161230197No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAGCGTACGACG410.11940820130475302No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG400.11649580615097856No Hit
CTAACCCCGAAACAACCAAACCCCACACGCCCTCGCTAAAACGTACGACG400.11649580615097856No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTGCGACG400.11649580615097856No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACAACG400.11649580615097856No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACA370.10775862068965517No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG360.1048462255358807No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAGACGTACGACG350.10193383038210624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCT100.00598788153.20801145
GACTCTT100.00598788153.20801145
CGACACA100.00706932144.944618
GACACAA100.00706932144.944619
TAACTCC151.2216132E-4144.944612
CCCCGAC100.00706932144.944615
CACCCCG450.0144.944613
CTAACTC151.2216132E-4144.944611
CTAACCC33250.0144.944611
CCCGACA100.00706932144.944616
CCCCCGA151.2216132E-4144.944614
TCACCCC450.0144.944612
TACCCCC100.00706932144.944612
CTCACCC450.0144.944611
TCCGAAA151.2216132E-4144.944616
CCGACAC100.00706932144.944617
CTACCCC100.00706932144.944611
TAACCCC33250.0144.50872
GTCTCTT33600.0144.31647145
CCGAAAC33850.0144.302327
Produced by FastQC (version 0.11.5)