FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11219
Sequences flagged as poor quality0
Sequence length150-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT540848.20393974507532No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT270524.110883322934306No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT8447.52295213477137No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT1971.7559497281397627No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT1891.6846421249665746No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT830.739816382921829No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT810.721989482128532No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTTGTGCGGT520.4634994206257242No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGC440.39219181745253584No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT430.38327836705588736No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTTGTGCGGT380.3387111150726446No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTTGTGCGGT360.32088421427934755No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT320.28523041269275334No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTTGTGCGGT270.24066316070951066No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGTGTGCGGT270.24066316070951066No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTTGTGCGGT250.22283625991621356No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCTGTGCGGT240.21392280951956505No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTTGTGCGGT230.20500935912291648No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTTGTGCGGT220.19609590872626792No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT220.19609590872626792No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT220.19609590872626792No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT200.17826900793297085No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTTGTGCGGT200.17826900793297085No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTTGTGCGGT190.1693555575363223No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT180.16044210713967377No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGCGCGGT180.16044210713967377No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT170.15152865674302524No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTCGTGCGGT170.15152865674302524No Hit
GAAGATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT150.13370175594972813No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT140.12478830555307958No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTTGTGCGGT140.12478830555307958No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT140.12478830555307958No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC140.12478830555307958No Hit
TAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT130.11587485515643105No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAGTTTTGTGCGGT130.11587485515643105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCTT201.8218325E-6148.28767145
TCTCTTT100.0065424438148.28767145
AAATTTA11000.0144.977683
ATAAGAT11100.0144.977689
AATTTAT11050.0144.977684
GAAAATT11000.0144.977681
TATAAGA11150.0144.977688
TTATAAG11100.0144.977687
AAAATTT11000.0144.977682
TTTATAA11100.0144.977686
ATTTATA11100.0144.977685
GTCTCTT10750.0142.76999145
TTTACGT8600.028.99553975-79
TTTTGTG7750.028.99553740-44
TTGTTTT403.1669333E-728.99553770-74
GTATGTT502.3464963E-928.99553795-99
GTAGAGT10700.028.99553755-59
GGTTGTA353.6960519E-628.99553750-54
TGGTTAG502.3464963E-928.99553745-49
GTTTGGA1550.028.99553730-34
Produced by FastQC (version 0.11.5)