FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11219
Sequences flagged as poor quality0
Sequence length150-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG601453.60549068544434No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG6195.5174257955254475No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG4714.198235136821463No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG4283.814956769765576No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA3142.7988234245476424No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG3062.727515821374454No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG1811.6133345217933863No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG1651.4707193154470095No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG1391.2389696051341474No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG1000.8913450396648542No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG620.5526339245922097No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG590.5258935734022641No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG570.508066672608967No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG520.4634994206257242No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACG520.4634994206257242No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG510.4545859702290757No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG470.41893216864248145No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACATACGACG450.40110526784918443No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG390.34762456546929316No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA360.32088421427934755No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG310.27631696229610486No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG240.21392280951956505No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG230.20500935912291648No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACA220.19609590872626792No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG220.19609590872626792No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACG210.18718245832961938No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT210.18718245832961938No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG210.18718245832961938No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA200.17826900793297085No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG200.17826900793297085No Hit
CTAACCCCGAAACAACCAGACTCCACACGCCCTCGCTAAAACGTACGACG190.1693555575363223No Hit
CTAACCCCGAAACAACCAGACTCCGCACACCCTCGCTAAAACGTACGACG190.1693555575363223No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACAACG190.1693555575363223No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACG180.16044210713967377No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACA180.16044210713967377No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG180.16044210713967377No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG180.16044210713967377No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA160.14261520634637667No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG160.14261520634637667No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACA150.13370175594972813No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG150.13370175594972813No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACATACGACG150.13370175594972813No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT140.12478830555307958No Hit
CTAACCCCGAAACACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT130.11587485515643105No Hit
CTAACCCCGAAACAACAAACTCCGCACGCCCTCGCTAAAACGTACGACGT130.11587485515643105No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG120.10696140475978252No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG120.10696140475978252No Hit
CGAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG120.10696140475978252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCC100.007001406144.977682
CTGACCC100.007001406144.977681
TAACCCC10950.0144.977682
CCCCGAA11050.0144.977685
CTAACCC10950.0144.977681
ACCCCGA11050.0144.977684
GACCCCG100.007001406144.977683
TTAACCC100.007001406144.977681
CCCGAAA11050.0144.321676
CGAAACA11050.0144.321678
CCGAAAC11050.0144.321677
AACCCCG11000.0144.318683
GAAACAA10950.0144.315679
GTCTCTT10950.0142.87077145
CGTAAAA8600.028.99553960-64
CAAACGT8600.028.99553955-59
GCCCTCA601.8189894E-1128.99553725-29
AAACCTT1500.028.995537105-109
ACACGCC900.028.99553725-29
AACAACC10850.028.99553710-14
Produced by FastQC (version 0.11.5)