FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13145
Sequences flagged as poor quality0
Sequence length148-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT861765.55344237352607No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT212616.17344998098136No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT2251.7116774438950173No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT1831.392164321034614No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTTGTGCGGT870.6618486116394066No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT710.5401293267402054No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGC650.49448459490300495No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTTGTGCGGT560.42601749714720427No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTTGTGCGGT540.4108025865348041No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTTGTGCGGT470.35755039939140354No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCTGTGCGGT450.34233548877900344No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTTGTGCGGT360.2738683910232027No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT360.2738683910232027No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTTGTGCGGT360.2738683910232027No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT350.26626093571700266No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGTGTGCGGT350.26626093571700266No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT320.24343856979840242No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT310.23583111449220237No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGCGCGGT310.23583111449220237No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT310.23583111449220237No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTTGTGCGGT300.2282236591860023No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTTGTGCGGT290.2206162038798022No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTTGTGCGGT220.16736401673640167No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTCGTGCGGT210.1597565614302016No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT200.15214910612400154No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAGTTTTGTGCGGT190.14454165081780143No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT160.12171928489920121No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTTGTGCGGT160.12171928489920121No Hit
GAAAATTTATAAGATTGCGTAAAGTTGCAAGGTTCGGAGTTTTGTGCGGT150.11411182959300115No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT150.11411182959300115No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT150.11411182959300115No Hit
GAAAATTTATAAGATTGCGTAAAGTAGTAAGGTTCGGTGTTTTGTGCGGT140.10650437428680107No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTGGTTGTTT140.10650437428680107No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTTGTGCGGT140.10650437428680107No Hit
GAAAATTTATAAGATTGTGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT140.10650437428680107No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC140.10650437428680107No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGTGTTTTGTGCGGT140.10650437428680107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTT12550.0145.36511145
TAAATTT151.2037033E-4144.969472
AATTTAC100.0070161605144.969474
GAAAATT12700.0144.969471
TTACAAG100.0070161605144.969477
TACAAGA100.0070161605144.969478
AAAATTT12700.0144.969472
ACAAGAT100.0070161605144.969479
TTTACAA100.0070161605144.969476
GTAAATT151.2037033E-4144.969471
ATTTACA100.0070161605144.969475
AAATTTA12900.0144.407583
TTTATAA12850.0144.405386
ATTTATA12850.0144.405385
AATTTAT12800.0144.403184
ATAAGAT12850.0143.84139
TATAAGA12850.0143.84138
TTATAAG12900.0143.283787
CGTTTGA12150.028.99389580-84
GGTTGTT12900.028.993895125-129
Produced by FastQC (version 0.11.5)