FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13145
Sequences flagged as poor quality0
Sequence length132-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG522739.7641688855078No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG371728.27691137314568No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG3452.6245720806390263No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG2161.6432103461392165No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG2121.6127805249144163No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG1471.1182959300114113No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACATACGACG1401.0650437428680106No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG1280.9737542791936097No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA1270.9661468238874097No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG1260.9585393685812096No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACA1240.9433244579688095No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACATACGACG990.7531380753138075No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG860.6542411563332066No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACG840.6390262457208064No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACATACGACG600.4564473183720046No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG530.40319513122860406No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG520.3955876759224039No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACATACGACG410.3119056675542031No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG400.3042982122480031No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG400.3042982122480031No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG370.2814758463294028No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATGCGACG370.2814758463294028No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG290.2206162038798022No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA280.21300874857360214No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG270.20540129326740206No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCATACGACG260.19779383796120195No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG250.1901863826550019No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT240.18257892734880182No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACACACGACG230.17497147204260174No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACAACG230.17497147204260174No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG220.16736401673640167No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG210.1597565614302016No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACGACG200.15214910612400154No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACA180.13693419551160135No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGGCG170.1293267402054013No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACATACGACG170.1293267402054013No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG170.1293267402054013No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG170.1293267402054013No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACGACG160.12171928489920121No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG160.12171928489920121No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG150.11411182959300115No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACATACGACGT140.10650437428680107No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG140.10650437428680107No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT140.10650437428680107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCTT151.0798064E-4148.95294145
TCTCTTT100.0064679193148.95294145
GAGGCAA100.0070156087144.973289
CCCAGAA100.0070156087144.973285
TGACCCC100.0070156087144.973282
GAAGCAA151.203577E-4144.973289
AACCCTG100.0070156087144.973283
CGAAGCA100.0070156087144.973288
CCTGAAA100.0070156087144.973286
AACCCAG100.0070156087144.973283
CTGACCC100.0070156087144.973281
ATAACCC100.0070156087144.973281
CGAGGCA100.0070156087144.973288
TAACCCT100.0070156087144.973282
CCCCGAG253.5122866E-8144.973285
GAACCCA100.0070156087144.973282
AGAAACA100.0070156087144.973288
CTAACCC12450.0144.973281
CGAACCC100.0070156087144.973281
CCCGAGG100.0070156087144.973286
Produced by FastQC (version 0.11.5)