FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21647
Sequences flagged as poor quality0
Sequence length148-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT1557771.95916293250797No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT20449.442416963089574No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT7263.3538134614496236No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT5342.466854529496004No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTTGTGCGGT1240.5728276435533792No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTTGTGCGGT980.4527186215179933No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTTGTGCGGT900.4157619993532591No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGC790.3649466438767497No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT650.3002725550884649No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTTGTGCGGT610.28179424400609787No Hit
GTAAATTTATAAGATTGCGTGAAGTTGTAAGGTTCGGAGTTTTGTGCGGT570.2633159329237308No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT550.25407677738254725No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTTGTGCGGT540.24945719961195545No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTTGTGCGGT530.2448376218413637No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCTGTGCGGT490.22635931075899665No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGGTTTGTGCGGT480.22173973298840488No Hit
GTAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT450.20788099967662954No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT430.198641844135446No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT430.198641844135446No Hit
GTTGACATAGGACTTTCCGAGAAGTAGTAACGTTAGGTGGGGTGTGCGGT420.19402226636485426No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTTGTGCGGT390.18016353305307897No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTTGTGCGGT370.1709243775118954No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGCGCGGT370.1709243775118954No Hit
GAAAATTTATAAGATTCCGTAAAGTAGTAAGGTTCGGTGTTTTGTGCGGT340.15706564420012012No Hit
GAAAATCTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT320.14782648865893658No Hit
GAAAATTTATAAGATTACGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT300.13858733311775304No Hit
TAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT270.12472859980597772No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTTGTGCGGT270.12472859980597772No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAGTTTTGTGCGGT250.1154894442647942No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT240.11086986649420244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT253.1648597E-8148.06383145
GTTGACA253.5914127E-8144.979171
TAGGACT253.5914127E-8144.979178
AAATTTA21150.0144.979173
AGGACTT253.5914127E-8144.979179
AATTTAT21150.0144.979174
GAAAATT21250.0144.979171
TGACATA253.5914127E-8144.979173
GACATAG253.5914127E-8144.979174
AAAATTT21150.0144.979172
TTTATAA21200.0144.979176
ATTTATA21200.0144.979175
ATAAGAT21300.0144.638849
TATAAGA21250.0144.638058
GTCTCTT21050.0144.19518145
TTATAAG21350.0143.960577
ATAGGAC301.06618245E-7120.815977
ACATAGG301.06618245E-7120.815975
TTGACAT301.06618245E-7120.815972
CATAGGA301.06618245E-7120.815976
Produced by FastQC (version 0.11.5)