FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21647
Sequences flagged as poor quality0
Sequence length149-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1124551.94715203030443No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA13596.278006190234213No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG12585.811428835404444No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG12435.742135168845568No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG6272.8964752621610383No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG5092.35136508523121No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACG2981.3766341756363467No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG2601.2010902203538596No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG2531.1687531759597172No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA2030.9377742874301289No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1160.5358710213886451No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG1060.4896752436827274No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACA960.44347946597680976No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACGACG830.3834249549591167No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACA810.37418579941793323No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG710.3279900217120155No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA680.31413128840024024No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG670.30951171062964844No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG640.29565297731787316No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG600.2771746662355061No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG550.25407677738254725No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACA540.24945719961195545No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACATACGACG490.22635931075899665No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG470.2171201552178131No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACA440.20326142190603777No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG440.20326142190603777No Hit
CTAACCCCGAAACAACCAGACTCCGCACACCCTCGCTAAAACGTACGACG400.18478311082367072No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG400.18478311082367072No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACAACG370.1709243775118954No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACG370.1709243775118954No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG370.1709243775118954No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGGACGACG350.1616852219707119No Hit
CTAACCCCGAGACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG350.1616852219707119No Hit
CTAACCCCGAAACAACCAGACTCCACACGCCCTCGCTAAAACGTACGACG330.15244606642952832No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT330.15244606642952832No Hit
CGAACCCAGAGGCAACCAAAGTCCGCACGCCCTCGCTAAAACGTACGACG330.15244606642952832No Hit
CTAACCCCGAAACAACCAGACTCCGCACGCCCTCGCTAAAACGTACGACA320.14782648865893658No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG300.13858733311775304No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG300.13858733311775304No Hit
CTAACCCCGAAACAACCAAACTCCGCGCGCCCTCGCTAAAACGTACGACG260.12010902203538597No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACAACG240.11086986649420244No Hit
CTAACCCCGAAGCAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG230.10625028872361066No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT230.10625028872361066No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG220.10163071095301889No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG220.10163071095301889No Hit
CTAACCCCGAAACAATCAAACTCCGCACGCCCTCGCTAAAACGTACGACG220.10163071095301889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCTT151.11621965E-4148.06383145
GAGGCAA253.5914127E-8144.979179
TAAACAA100.0070462725144.979179
CCCAGAG151.2142344E-4144.979175
GAAACAA20800.0144.979179
TGACCCC306.220944E-10144.979172
CCTAAAC100.0070462725144.979177
GAAGCAA100.0070462725144.979179
AGAGGCA253.5914127E-8144.979178
CCAGAGG100.0070462725144.979176
CGAAGCA100.0070462725144.979178
AACCCCT100.0070462725144.979173
AACCCAG100.0070462725144.979173
CTGACCC306.220944E-10144.979171
CAGAGGC253.5914127E-8144.979177
CAGAGAC100.0070462725144.979177
TAACCCC20600.0144.979172
CCCCGAA20950.0144.979175
GAACCCC151.2142344E-4144.979172
GAACCCA100.0070462725144.979172
Produced by FastQC (version 0.11.5)