FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11843449
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG36350.025.5328083
CTACTCG41650.024.3728352
AGTAGCT151550.024.3051855
TACTCGG45600.023.8517043
AAAGTGC224100.023.781364
GCTGGGA413800.023.1090099
CCGAGTA40600.022.6814442
TAGCTGG175450.022.4373537
CCAAAGT260950.021.9792142
AAGTGCT247500.021.6501265
GAGTAGC99950.021.4733644
CCCAAAG307400.021.1821961
GTAGCTG172600.021.0046236
GCTACTC108100.020.5255241
CAAAGTG274450.020.5017763
CGTCGAG18250.018.6738341
AGTGCTG291400.018.3882566
GCTACTT98250.018.08141
CTACTTG90900.017.788282
CCCGAGT64800.016.7847461
Produced by FastQC (version 0.11.5)