FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11843449
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127980.10805973834142402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG36450.031.4198072
TACTCGG40550.027.158393
CGAGTAG39250.024.9198513
GCTACTC105900.024.60091
AAAGTGC224450.023.8907324
CCGAGTA41750.023.0908072
CCAAAGT259550.022.7325062
GCTGGGA414550.022.0857729
AGTAGCT148050.022.0263045
AAGTGCT250550.021.8079325
CCCAAAG303900.021.7301751
CAAAGTG266650.021.3566063
TAGCTGG170250.021.2446737
GAGTAGC101000.020.08884
GTAGCTG168400.019.9266056
AGTGCTG300400.018.5050326
GCTACTT96350.018.0010851
CCCGAGT66900.017.8982431
ACTCGGG67500.017.4985144
CTACTTG89900.017.3349552
Produced by FastQC (version 0.11.5)