FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11899049
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG36900.021.0269572
TACTCGG42850.018.9532783
CCAAAGT209350.018.808142
AAAGTGC183600.018.7602274
TACATCG33300.017.635977145
CGAGTAG32950.017.6056353
GCTACTC91900.017.5985831
CAAAGTG220700.017.1836933
CCCAAAG276750.016.6408081
AGTAGCT112600.016.5506695
AAGTGCT208200.016.4044255
GTCGAGA29000.016.2556271
CGTCGAG24600.015.9201441
GCTGGGA394200.015.3572059
GAGTAGC81050.015.2989024
TAGCTGG135050.015.2462927
GACTTGT88200.013.8942553
CCGAGTA40800.013.6851482
GCTAATT86400.013.4305741
ACTTGTG99250.013.3701944
Produced by FastQC (version 0.11.5)