FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11899049
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165440.13903632130601362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA10400.021.610453145
AAAGTGC185900.020.4721034
CTACTCG35400.020.0683962
CCAAAGT214600.019.2884032
GTCTCGC64550.019.20593145
GCTACTC85700.018.3652571
TACTCGG41150.018.1445523
CAAAGTG224450.018.0539023
AAGTGCT212250.017.793665
CCCAAAG274000.017.6825141
AGTAGCT114150.017.1460115
CGAGTAG37000.017.0449773
GCTGGGA399650.016.1805449
GCTACTT79850.015.986591
CTACTTG71800.015.8513082
TAGCTGG137050.015.8154017
CCGAGTA42400.015.5584162
GAGTAGC83700.014.290294
AGTGCTG286700.013.8810326
GCTATAT40450.012.73086551
Produced by FastQC (version 0.11.5)