FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11245801
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG21800.028.9460491
ACGTCGA12900.022.4903811
GTCGAGA28050.022.2283232
CGAGTAG32950.022.0029963
AAAGTGC196650.021.4568654
CCGAGTA35000.020.7144362
CTACTCG32550.020.4916972
CCAAAGT223100.020.375542
AGTAGCT122450.019.8935345
TAGCTGG139100.019.8578057
TACTCGG35700.019.6988453
AAGTGCT220950.019.0967945
GCTGGGA373750.018.9906029
GCTACTC86700.018.4884151
CAAAGTG236400.018.3703143
CCCAAAG271850.018.329641
GAGTAGC84650.018.2427334
GTAGCTG148300.016.670356
GCTACTT83100.016.4090461
CTACTTG82650.016.1404672
Produced by FastQC (version 0.11.5)