FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11245801
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG37250.026.2671992
TACTCGG39250.024.9235323
AAAGTGC186600.022.9140684
CCAAAGT222650.021.97292
GCTACTC92300.021.534651
AGTAGCT119650.019.9898475
CAAAGTG234250.019.9833563
AAGTGCT218400.019.74575
CGAGTAG32300.019.7422053
CCCAAAG275500.019.7219181
GCTGGGA360950.019.6440689
CCGAGTA34650.019.0346322
TAGCTGG140750.018.0255997
GAGTAGC82700.017.7014054
CTACTTG81550.016.7086052
GCTACTT86700.016.4829941
AGTGCTG274050.016.159916
TTGGGAG209350.016.099326
TCGGGAG67450.016.0114946
GTCTCGC45000.015.7880335145
Produced by FastQC (version 0.11.5)