FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12021100
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG36550.025.9878032
CGAGTAG35100.023.5493973
CGTCGAG19600.022.5699121
TACTCGG41550.022.5113073
CCGAGTA38950.022.1526242
GCTACTC98600.020.5938361
AAAGTGC203900.020.4471444
CCAAAGT237400.019.3945052
AGTAGCT133800.018.8042245
GCTGGGA393700.018.2125029
TAGCTGG154100.018.0662177
CAAAGTG250250.017.9928343
CCCAAAG286650.017.9623241
AAGTGCT233550.017.8202615
TCTCGTT41650.017.581646145
GAGTAGC93250.016.7175144
AGCATCG72150.016.279154145
GTCGAGA27250.016.2311382
GCTACTT91000.016.0180931
GTAGCTG161800.015.6845316
Produced by FastQC (version 0.11.5)