FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12021100
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150640.12531299132358936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC203250.023.6813874
CTACTCG36550.022.412652
CCAAAGT240450.021.737652
GCTACTC103000.021.4065321
AAGTGCT231400.021.3325395
CGAGTAG33450.021.2370473
CCCAAAG290150.020.5975061
GCTGGGA400000.020.3707339
TACTCGG42200.020.2690643
AGTAGCT132650.020.0547665
CAAAGTG251500.020.0025633
CCGAGTA37150.019.7090032
TAGCTGG154250.018.1404487
GAGTAGC89250.017.8683344
AGTGCTG293450.016.9459866
GTAGCTG157650.016.2774416
GTCTCGC55200.015.497553145
GGAGGCT319500.014.31722459
GTGCTGG366300.014.1697437
TTGGGAG220500.013.9719546
Produced by FastQC (version 0.11.5)