FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12934014
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG43250.024.9665722
CGCGATA7850.023.090271145
AAAGTGC205700.023.0745834
CCAAAGT244500.021.9633262
CGAGTAG40300.021.216333
TACTCGG51850.020.82243
AAGTGCT236950.020.2780275
GCTACTC107350.020.0683381
CAAAGTG256100.020.0599563
CCCAAAG314700.019.2232281
AGTAGCT131050.018.3045835
CCGAGTA47900.017.8527572
GAGTAGC94900.017.5625864
GCTACTT90900.017.3960081
TAGCTGG155700.017.0402987
GCTGGGA450950.016.9298069
CTACTTG84450.016.7337682
AGTGCTG314900.015.4441526
GTAGCTG169250.014.0462186
TACTTGG106400.013.9606463
Produced by FastQC (version 0.11.5)