FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11500891
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG35800.027.3397642
CGAGTAG38150.026.9859543
CCGAGTA40400.025.841942
TACTCGG41300.025.2787973
AGTAGCT143900.024.2333325
AAAGTGC218100.023.4687124
GCTACTC100700.023.3359621
TAGCTGG165250.023.2961317
GCTGGGA398100.022.6730469
ACGTCGA11750.022.2215731
CCAAAGT253950.022.1257232
GAGTAGC99250.021.6953244
GTAGCTG165250.021.6727666
AAGTGCT245850.021.2025245
CAAAGTG261150.020.877183
CCCAAAG306700.020.5265581
CGTCGAG16950.019.2554191
AGTGCTG290600.018.4115186
GTCGAGA25350.018.3110051
GCTACTT92200.017.6995321
Produced by FastQC (version 0.11.5)