FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11500891
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG35100.030.9638823
CCGAGTA38850.028.5400522
CTACTCG40300.026.793882
TACTCGG45400.024.417823
GCTACTC106800.024.0438521
AAAGTGC215900.023.6612664
AGTAGCT145300.022.942915
CCAAAGT246450.022.9361322
GAGTAGC95450.022.4707364
GCTGGGA401300.021.5888169
CCCAAAG289750.021.4550441
AAGTGCT241500.021.3357935
CAAAGTG254250.021.2878063
TAGCTGG163300.021.2181537
GTAGCTG160050.019.653486
CCCGAGT63300.019.2520771
AGTGCTG285700.018.1384936
GGAGGCT310150.017.1808919
TTGGGAG234400.017.0372476
GCTACTT93200.016.8116321
Produced by FastQC (version 0.11.5)