FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12115036
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG40500.023.2729632
AAAGTGC201750.022.3167344
AGTAGCT129750.021.1776265
TACTCGG46350.021.117653
CCAAAGT232900.020.95122
GCTACTC95250.020.7928411
AAGTGCT228950.019.9817775
CGAGTAG37700.019.616453
TAGCTGG151850.019.28917
CCCAAAG294850.018.9454861
CAAAGTG247050.018.9000323
GCTGGGA424800.017.783169
CCGAGTA45400.017.7268052
GAGTAGC94650.017.4649284
GTAGCTG166400.016.3389196
CTACGCC45350.015.987041145
GCTACTT85200.015.071271
AGTGCTG302750.014.8953816
GTCGAGA25600.014.7274212
CTACTTG82250.014.3686192
Produced by FastQC (version 0.11.5)