FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13078209
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG41450.023.090882
CGAGTAG40150.022.2130073
TACTCGG47600.020.8689883
AGTAGCT135500.020.7624475
CCGAGTA45550.020.5348912
AAAGTGC208150.018.9848084
TAGCTGG162750.018.9767387
GCTACTC106100.018.7254771
CCAAAGT247150.018.4829392
GAGTAGC97550.017.6903254
GTCGAGA28600.017.4936641
AAGTGCT238800.017.2768295
CAAAGTG255850.016.9190793
GCTGGGA454150.016.5703249
CCCAAAG318750.016.4469661
GTAGCTG176950.015.8578096
GCTACTT90250.015.6669751
CTACTTG84300.014.880232
AGTGCTG314500.013.23347956
CAAGTAG81500.013.1671523
Produced by FastQC (version 0.11.5)