FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13078209
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142490.1089522273271516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC215150.022.9042364
CCAAAGT248250.021.9316732
CTACTCG41900.021.455012
AAGTGCT245900.020.1001365
CAAAGTG261900.019.6427823
CGAGTAG37000.019.3871353
CCCAAAG320200.019.2407991
TACTCGG48950.018.2067553
CTACTTG82900.017.8400782
AGTAGCT133750.017.8268765
GCTGGGA459750.017.5181589
GCTACTT90700.017.3615781
CCGAGTA43900.017.0095882
GCTACTC105200.016.9689941
TAGCTGG159100.016.8569057
AGTGCTG318950.015.7024296
CGCGATA6951.6189006E-1015.646453145
GAGTAGC99900.015.3786654
GTCTCGC60100.014.47492145
GTAGCTG170950.014.2456276
Produced by FastQC (version 0.11.5)