FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11413573
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA17300.026.8312241
AAAGTGC174500.020.6072334
CTACTCG35100.019.2095382
CGTCGAG26650.018.771242
CCAAAGT203200.018.5889592
GCTACTC82950.018.0118141
CAAAGTG206100.017.940133
AAGTGCT199250.017.9380955
AGTAGCT107650.017.779425
TAGCTGG130000.017.4554337
CGAGTAG31450.017.2891673
CCCAAAG261700.016.767111
TACATCG31800.016.645605145
GCTGGGA385850.016.2715559
GTCGAGA30300.016.0311853
TACTCGG41350.015.4291013
CCGAGTA41000.015.0305932
GAGTAGC81650.014.7395874
CTACTTG69650.014.364782
GCTACTT73850.014.1422531
Produced by FastQC (version 0.11.5)