FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11413573
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA9200.030.732796145
CTACTCG35300.023.4044972
TACTCGG44150.020.1873283
AAAGTGC175950.018.863184
CCAAAGT200400.018.660412
GCTACTC87100.018.4038581
GTCTCGC63350.018.195993145
CCCAAAG256650.017.3524931
CAAAGTG211150.016.9870743
AAGTGCT200550.016.8038585
AGTAGCT106350.016.5595075
CGAGTAG32950.016.053583
GCTGGGA379150.015.1823339
TAGCTGG127100.015.1140827
GCTACTT73900.014.035451
GCTAATT78800.013.6229221
CTACTTG69600.013.5364032
CCGAGTA39650.013.5256362
GAGTAGC81300.013.1028414
AGTGCTG270600.012.8568336
Produced by FastQC (version 0.11.5)