FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11575854
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32200.026.1219233
CTACTCG32100.025.751522
TACTCGG36850.023.4159873
CCGAGTA38050.021.724672
AGTAGCT130300.020.9796415
GCTACTC94750.020.2038141
AAAGTGC196100.020.1891524
TAGCTGG146600.019.979167
CCAAAGT226400.019.8251862
CGTCGAG19150.019.6898861
GAGTAGC90950.018.8950754
GTCGAGA26250.018.2315351
AAGTGCT227000.018.1755495
GCTGGGA385900.018.03559
GTAGCTG152950.018.014736
CAAAGTG237900.017.708623
CCCAAAG278100.017.6781711
GCTACTT84650.016.3611951
CCCGAGT63400.015.7833221
CTACTTG82450.014.6868542
Produced by FastQC (version 0.11.5)