FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11575854
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140950.1217620747462779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG36050.028.357292
CGAGTAG31450.024.1927283
TACTCGG43700.023.2146873
AAAGTGC194200.022.539164
GCTACTC96150.022.117071
CCGAGTA35150.022.0703562
CCAAAGT226600.021.4370862
GCTGGGA374100.020.2698159
AGTAGCT123100.020.2523235
AAGTGCT227000.019.6985245
TAGCTGG140550.019.3911117
CAAAGTG236850.019.3662953
CCCAAAG277200.019.3490851
GAGTAGC86150.017.3285394
GTAGCTG149050.016.7276386
TCGGGAG74100.016.4323356
GTCTCGC48600.016.110544145
ACTCGGG70000.015.8395714
AGTGCTG279700.015.7550346
GGAGGCT306600.015.6764319
Produced by FastQC (version 0.11.5)