FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13187821
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG40950.024.2565962
GCTACTC105200.021.5156291
CGAGTAG41150.020.9669553
TACTCGG50100.020.4051443
AAAGTGC215450.019.3835184
CCGAGTA47950.019.05222
CCAAAGT243550.019.0228632
AGTAGCT138050.018.8013945
AAGTGCT246200.017.3743025
CAAAGTG260300.017.3529013
TAGCTGG165450.017.0462137
CCCAAAG317400.016.8223291
GTCGAGA30750.016.278681
GAGTAGC100850.016.0319214
GCTGGGA462300.016.0271859
GTAGCTG175500.015.3677136
CGTCGAG26950.015.3437421
AGGATCG59300.015.038131145
GCTACTT93850.014.918951
ACGTCGA18300.014.6678291
Produced by FastQC (version 0.11.5)