FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13187821
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG44450.021.5266592
TACTCGG51300.019.64133
CCAAAGT248050.019.5214332
AAAGTGC215100.019.5126324
GCTACTC106000.019.4282421
AGTAGCT137200.019.0210465
TAGCTGG158800.018.899737
CGAGTAG39750.018.600983
CAAAGTG256700.018.1858163
CCCAAAG320000.017.539291
AAGTGCT245150.017.4464115
GAGTAGC97250.017.293234
CCGAGTA45850.016.7587242
GCTGGGA467050.016.593769
GCTACTT92950.016.070851
CTACTTG85600.015.8358842
GTAGCTG176600.015.3523586
AGTGCTG320300.013.8965076
GGAGGCT380550.013.6024449
TCGGGAG98550.013.2406736
Produced by FastQC (version 0.11.5)