FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12315929
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135870.1103205450437397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG38550.027.4539263
CTACTCG43300.026.787322
CCGAGTA43150.025.2004092
TACTCGG47700.024.1632163
AAAGTGC227950.023.1510834
AGTAGCT155350.022.117595
GCTACTC114350.022.0731471
GCTGGGA424200.022.028499
CCAAAGT265800.021.7915942
TAGCTGG175850.021.7662417
AAGTGCT253050.020.9402435
CAAAGTG275200.020.3613723
CCCAAAG318300.020.3258841
GTAGCTG172600.019.781686
GAGTAGC105850.019.4494364
CTACTTG96700.019.1166112
GCTACTT99400.018.6069431
CCCGAGT70250.017.9648721
TTGGGAG251050.017.7004096
AGTGCTG305500.017.6777956
Produced by FastQC (version 0.11.5)