FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9622322
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA14400.028.20671
CGTCGAG21600.024.5129641
GTCGAGA26850.019.4419082
AAAGTGC141800.018.5596494
CTACTCG26400.017.5762712
CCAAAGT171650.017.1910062
AGTAGCT87650.016.4599785
GCTACTC69200.016.036591
AAGTGCT167800.015.8995185
CAAAGTG178700.015.8633943
CCCAAAG226500.015.6270981
GCTGGGA312450.015.1055089
GACTTGT80950.014.5987153
TAGCTGG106750.014.2620447
ACTTGTG94500.014.0397274
GCTAATT73900.013.8388951
TCGAGAC40450.013.8011763
CGAGTAG25300.013.7551263
TACTCGG32050.013.1203023
GCTACTT63750.013.0841151
Produced by FastQC (version 0.11.5)