FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9622322
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG27850.022.1182582
AAAGTGC149750.019.6949754
GCTACTC70150.018.9237631
CCAAAGT172150.018.5226382
TACTCGG32750.018.1415313
AGTAGCT93850.017.3750365
AAGTGCT173300.017.062365
CAAAGTG182800.016.6869643
CGAGTAG24850.016.6195743
CCCAAAG224100.016.3144631
GTCTCGC43600.016.128658145
GCTGGGA317600.015.6825379
TAGCTGG112750.015.2369277
CCGAGTA30000.014.4939642
GAGTAGC68050.014.3758474
GTAGCTG124250.013.4748046
TCGACGG17950.013.327924145
GCTACTT64250.013.32272151
AGTGCTG230800.013.2834666
CTACTTG61550.012.1273622
Produced by FastQC (version 0.11.5)