FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9909224
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG21850.024.2373981
ACGTCGA12300.020.6432251
GTCGAGA26800.020.290222
AAAGTGC153100.018.6111534
GCTACTC72700.017.862131
CCAAAGT179950.017.808642
AAGTGCT176900.016.3528045
CAAAGTG191750.016.1453863
CTACTCG26800.015.961642
CCCAAAG230300.015.7188661
AGTAGCT93450.015.6718135
CGAGTAG29900.014.5490943
GCTGGGA328650.014.5152359
TAGCTGG114750.014.026587
CCGAGTA33250.013.7375482
TCGAGAC43200.013.4264793
CGAGACT55150.013.154341
GAGTAGC64500.013.0392974
AGTGCTG231900.012.8808236
TACTCGG32900.012.5613083
Produced by FastQC (version 0.11.5)