FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9909224
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104370.10532610828052731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTC71550.019.6595211
CTACTCG31250.018.787882
AAAGTGC153550.018.504574
CGAGTAG28700.018.4366283
TACTCGG36450.017.4995023
CCAAAGT176300.017.2679082
CCGAGTA32650.017.0942572
AAGTGCT175500.016.769085
CAAAGTG185600.016.2463533
GTCTCGC43900.016.18431145
AGTAGCT95600.015.9228825
CCCAAAG223950.015.7349751
GCTGGGA322100.014.8778089
GAGTAGC69300.014.5386584
TAGCTGG112700.014.215127
TCGACGG18150.013.581098145
GCTACTT65250.013.3346491
CCCGAGT56750.013.2876541
GCTAATT73100.013.0929471
AGTGCTG236250.012.8254116
Produced by FastQC (version 0.11.5)