FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11875706
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG39400.029.0752832
CGAGTAG37100.025.9918483
TACTCGG45300.025.1281723
AAAGTGC226050.023.5421144
ACGTCGA11250.023.2146341
GCTACTC106900.022.7341631
CCGAGTA42500.022.348352
AGTAGCT153950.022.0401545
CCAAAGT262300.021.6987382
GCTGGGA421400.021.5224199
TAGCTGG176400.021.1668037
AAGTGCT253450.020.9681515
CCCAAAG311350.020.5742361
CAAAGTG268350.020.5068823
GAGTAGC104800.020.062764
GTAGCTG169800.019.8974176
AGTGCTG295650.018.1714176
TCGGGAG73400.017.483396
TTGGGAG244600.016.687856
CCCGAGT69100.016.5878811
Produced by FastQC (version 0.11.5)