FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11631540
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG35300.026.495092
CGAGTAG34800.025.0005023
TACTCGG39950.023.5924633
CGTCGAG22200.023.2015651
CCGAGTA38700.023.0432932
AAAGTGC199900.021.2171924
GCTACTC93550.021.170481
AGTAGCT132900.021.0026915
ACGTCGA13900.020.8764691
TAGCTGG151550.020.7144327
GCTGGGA386550.019.786689
CCAAAGT238250.019.5976222
AAGTGCT223300.019.1558975
CAAAGTG246000.018.5379733
GAGTAGC90850.018.3547174
GTAGCTG156850.018.257946
CCCAAAG288950.018.127021
GTCGAGA30350.017.1998312
TCTCGTT42400.016.757122145
AGTGCTG276800.015.8725166
Produced by FastQC (version 0.11.5)